No, I can't really edit stuff out of the ISO, as if that were possible, it would've been documented on one of these forum sites. But yes, the mods work perfectly fine. And I do know that a user also requested an unlocked BIOS as well, it's just the he/she knows all the options he/she wants.

@SamsungASUS - I wasn't sure if there was ISO editing tools for 2000, like there is for XP, Win7-10 etc. Thanks for confirmation BIOS is all OK.
@vickyk1293 is who I was talking about also wanting this unlocked, I will merge their thread here later - [REQUEST] Acer Aspire one zg5(AOA150) Insydeh20 Rev 3.5 Bios v3310 Advance Menu option Unlock
Whoever you are talking about, if not the above user, you can link them to this thread. That BIOS above at post #24 is 99.99% all settings visible, so anything specific someone wants will be included in that BIOS

Hi @Lost_N_BIOS. Thanks for getting back to me. Sorry that my post is confusing. I don't remember if NX bit setting and the AHCI setting are hidden in the original BIOS or not as it has been a while since i last used the machine. I have downloaded the unlocked bios you posted in the thread Acer Aspire One AOA150 BIOS Mod at post #24, but the pci options are still hidden in that.What i wanted was to get the 'PCI Configuration' to be made visible under the Advanced tab as I wanted to attach a SATA controller to the mini pcie port which will give me two extra SATA ports to use. The controller is based on Asmedia ASM-1061 and is quite popular one on ebay, but i have read that it is very much dependent on bios for proper initilization. Currently the card doesn't show up on boot and neither does the kernel see it in the os. Might be the bios has a whitelist for the type of cards that can be used in the mini pcie port. Any idea how to make the bios recognize and initialize the card?

@vickyk1293 - It's OK, all sorted now And no worries about those settings, I had user send me images post-unlock, and then I did follow-up edit to make sure all remaining settings were also revealed, so I've fully unlocked this BIOS now and it's user tested without issue.
You need to download follow-up BIOS probably, BIOS at post #24 has 100% of possible settings in the BIOS visible to you (minus what I mentioned). Please show me image of root of advanced, it's possible I missed that, he didn't send me new follow-up images post unlock
Ohh! I checked, and I see what's going on now with that. This is not a submenu contained/linked within advanced, so it can't be enabled like everything else, see below, see how the rest of the submenus are there directly under advanced root, PCI Config is not, so it's can't be put in/enabled

0x9A52 Form: Advanced, Form ID: 0x1 {01 06 01 00 53 00}
0x9A58 Subtitle: {02 04 2D 00}
0x9A5C Selectable: Boot Configuration, Form ID: 0x21 {0F 0B 21 00 55 00 56 00 00 00 00}
0x9A67 Selectable: Peripheral Configuration, Form ID: 0x22 {0F 0B 22 00 57 00 58 00 00 00 00}
0x9A72 Selectable: IDE Configuration, Form ID: 0x23 {0F 0B 23 00 59 00 5A 00 00 00 00}
0x9A7D Selectable: Video Configuration, Form ID: 0x25 {0F 0B 25 00 5D 00 5E 00 00 00 00}
0x9A88 Selectable: USB Configuration, Form ID: 0x26 {0F 0B 26 00 5F 00 60 00 00 00 00}
0x9A93 Selectable: Chipset Configuration, Form ID: 0x27 {0F 0B 27 00 61 00 62 00 00 00 00}
0x9A9E Selectable: ACPI Table/Features Control, Form ID: 0x28 {0F 0B 28 00 63 00 64 00 00 00 00}
0x9AA9 End Form {0B 02}
0x9AAB Form: InsydeH2O Setup Utility, Form ID: 0x20 {01 06 20 00 2B 00}
0x9AB1 Subtitle: PCI Configuration {02 04 54 00}

However, it may be possible that this shows up as settings only, inside advanced, without being inside a submenu when unsuppresed, but I doubt it since it is actually it's own submenu
Please test this BIOS and let me know - https://ufile.io/wqj41rsm
If it's still not there, then the only thing we can do is directly change the settings to the values you want. There is only two settings in there, shown below, let me know what you want them set to and I will make the change here and in NVRAM
If there was a whitelist blocking or only allowing certain cards, you will see error message at boot and would not be able to proceed until card was removed. It's possible this slot is not physically compatible with card you are trying to use, wrong or missing connections etc.

Setting: PCI Slot3 IRQ Priority, Variable: 0x2[1] {05 09 02 00 01 69 00 6B 00}
0x9AD5 Option: Auto, Value: 0x0 {09 09 30 00 00 00 05 41 00}
0x9ADE Option: IRQ3, Value: 0x3 {09 09 6C 00 03 00 04 41 00}
0x9AE7 Option: IRQ4, Value: 0x4 {09 09 6D 00 04 00 04 41 00}
0x9AF0 Option: IRQ5, Value: 0x5 {09 09 6E 00 05 00 04 41 00}
0x9AF9 Option: IRQ7, Value: 0x7 {09 09 6F 00 07 00 04 41 00}
0x9B02 Option: IRQ9, Value: 0x9 {09 09 70 00 09 00 04 41 00}
0x9B0B Option: IRQ10, Value: 0xA {09 09 71 00 0A 00 04 41 00}
0x9B14 Option: IRQ11, Value: 0xB {09 09 72 00 0B 00 04 41 00}
0x9B1D End of Options {10 02}
0x9B1F Setting: PCI Slot4 IRQ Priority, Variable: 0x3[1] {05 09 03 00 01 6A 00 6B 00}
0x9B28 Option: Auto, Value: 0x0 {09 09 30 00 00 00 05 41 00}
0x9B31 Option: IRQ3, Value: 0x3 {09 09 6C 00 03 00 04 41 00}
0x9B3A Option: IRQ4, Value: 0x4 {09 09 6D 00 04 00 04 41 00}
0x9B43 Option: IRQ5, Value: 0x5 {09 09 6E 00 05 00 04 41 00}
0x9B4C Option: IRQ7, Value: 0x7 {09 09 6F 00 07 00 04 41 00}
0x9B55 Option: IRQ9, Value: 0x9 {09 09 70 00 09 00 04 41 00}
0x9B5E Option: IRQ10, Value: 0xA {09 09 71 00 0A 00 04 41 00}
0x9B67 Option: IRQ11, Value: 0xB {09 09 72 00 0B 00 04 41 00}
0x9B70 End of Options {10 02}

Also, merging thread now

* Edit - I can possibly replace some other submenu inside advanced with this one, if you wanted? If yes, which submenu do you not need, or don't care if it's missing and replaced with PCI Config submenu instead?

Not trying to bud in, but I flashed the new bios, and the options aren't there. Sorry.

@SamsungASUS - Thanks for checking! So the only option for @vickyk1293 is to then directly set what she wants, or for her to pick a submenu for me to swap in PCI Config submenu instead.

You're very welcome. BTW, I sent you pictures of the other menus on the ASUS Prime B450M-A/CSM taken by the F12 screenshot. I also sent a reply about other options I need either visible or unlocked. Sorry for the inconvenience.

I'll check it out

Thanks.

@lost_n_bios thanks for all the help. Really appreciate it. I was away from the netbook so could not try the new bios. Now that I have access to, I have installed the bios mentioned in post #34 and the card is still not working. If possible can you put the PCI Config under advanced root by replacing the Boot Configuration submenu as it doesn't have much in it?

Thanks.

@vickyk1293 - It's OK, we're all busy in the world so never a rush. SamsungASUS tested for me already, so I knew it would not be there, was just waiting on you to pick something to replace so we can try to make it show that way.
If not, I can change either of those options to whatever you want, just let me know what you want them set to, if it doesn't show up that is
I am out of time for tonight, so I will have to get on this tomorrow, sorry. But I will get done soon as I can, since you've now given me item to replace

@Lost_N_BIOS could you replace the 'Boot Configuration submenu' with the PCIe options under the 'Advanced' root menu.

This PCI settings:
Setting: PCI Slot3 IRQ Priority, Variable: 0x2[1] {05 09 02 00 01 69 00 6B 00}
:
:
0x9B70 End of Options {10 02}

under 0x9A5C Selectable: Boot Configuration, Form ID: 0x21 {0F 0B 21 00 55 00 56 00 00 00 00}

Thanks

@vickyk1293 - Here, please test and let me know outcome. https://ufile.io/zotuxj5x
Is "Boot Config" submenu replaced with "PCI Config" submenu and you can enter it and see correct setting?
If not, what do you see in Boot Config's place, or it's simply missing, or you can't enter, or wrong stuff inside etc?

Good news. Just flashed the BIOS, and the PCI options show. But I'm also experimenting and trying to find the right PCI settings for Windows 2000. BTW, sorry, I'm not trying to nag or rush you, but I sent replies regarding the ASUS Prime B450-M bios.

@SamsungASUS - Thanks for testing to let us know!!
@vickyk1293 - Swap confirmed OK and working ^^